KMID : 0364820150510030209
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Korean Journal of Microbiology 2015 Volume.51 No. 3 p.209 ~ p.220
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Analysis of gut bacterial diversity and exploration of cellulose-degrading bacteria in xylophagous insects
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Choi Min-Young
Ahn Jae-Hyung Song Jae-Kyeong Kim Seong-Hyun Bae JIn-Woo Weon Hang-Yeon
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Abstract
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In this study, gut bacterial communities in xylophagous insects were analyzed using the pyrosequencing of 16S rRNA genesfor their potential biotechnological applications in lignocelluloses degradation. The result showed that operational taxonomic units(OTUs), species richness and diversity index were higher in the hindgut than in the midgut of all insect samples analyzed. The dominantphyla or classes were Firmicutes (54.0%), Bacteroidetes (14.5%), ¥ã-Proteobacteria (12.3%) in all xylophagous insects except for Rhinotermitidae. The principal coordinates analysis (PCoA) showed that the bacterial community structure mostly clustered according to phylogeny of hosts rather than their habitats. In our study, the two carboxymethyl cellulose (CMC)-degrading isolates which showed the highest enzyme activity were most closely related to Bacillus toyonensis BCT-7112T and Lactococcus lactis subsp. hordniae NCDO 2181T ,respectively. Cellulolytic enzyme activity analysis showed that ¥â-1,4-glucosidase, ¥â-1,4-endoglucanase and ¥â-1,4-xylanase were higher
in the hindgut of Cerambycidae. The results demonstrate that xylophagous insect guts harbor diverse gut bacteria, including valuable cellulolytic bacteria, which could be used for various biotechnological applications.
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KEYWORD
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cellulolytic bacteria, lignocellulose degradation, pyrosequencing, xylophagous insect
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